Dott.ssa Mariafrancesca SCALISE
 

 

Researcher of Biochemistry

SSD: BIO/10

DiBEST
Ponte Pietro Bucci
Cubo 4C – floor 4

PHONE: +39 0984/492938

EMAIL: mariafrancesca.scalise@unical.it

 

   RESEARCH INTERESTS
 

 

MEMBRANE TRANSPORTERS BIOCHEMISTRY:
- Protein purification by chromatographic techniques using FPLC application.
- Transport measurements by radioisotope and fluorescence techniques.
- Over-expression in human cells and transport assays in intact cells.
- Transport assays in artificial membrane system (proteoliposomes).
- Membrane transport mechanisms and structure/function relationships (homology modeling, site
- directed mutagenesis and chemical labeling).
- Evaluation of effects of endogenous and exogenous compounds
- Evaluation of involvement of the studied proteins in human pathologies: cancer, diabetes, inflammatory diseases.

 

MEMBRANE TRANSPORTERS UNDER INVESTIGATION:
- Amino acid transporters: hASCT2, hLAT1, hSLC38A9, SlCAT2.
- Sialic acid transporter: SiaT of pathogenic bacteria
- Organic cation transporters: OCTN1, OCTN2, OCTN3

 

 

   LABORATORIES

 

  - Unit of Biochemistry and Molecular Biotechnologies Cubo 4C IV floor;

 

 

   PUBBLICATIONS

 

 

   TEACHING COURSES

 

 - Applied Biochemistry and Molecular Biology - Master Degree in Biology